For Influenza Centres
Submitting Samples
Shipping Instructions
HI Typing Assay
Molecular Analysis


Contact
WHO Collaborating Centre for Reference and Research on Influenza (VIDRL)
Peter Doherty Institute for Infection and Immunity
792 Elizabeth Street
Melbourne VIC 3000 Australia

T +61 3 9342 9300
F +61 3 9342 9329
whoflu@influenzacentre.org
Home > For Influenza Centres > Molecular analysis

Molecular Analysis Resources

Sequencing primers
The Centre has developed two set of primers, 17 pairs for Type A and 19 pairs for Type B, that enable the full genomes of both Type A and Type B viruses to be sequenced for surveillance purposes. If you are from a National Influenza Centre and would like to access the sequences of these primers, please send us an email at whoflu@influenzacentre.org with the subject line "NIC request for primer sequences".

The Centre has published a paper describing a simple and streamlined method for sequencing the
full genome of influenza A viruses using Sanger sequencing. The paper, which contains full details of the
method and primer sequences used, is freely accessible:

Deng YM, Spirason N, Iannello P, Jelley L, Lau H and Barr IG. A simplified Sanger sequencing method for full genome sequencing of multiple subtypes of human influenza A viruses. J Clin Virol. DOI: 10.1016/j.jcv.2015.04.019
Access the paper here

A(H7N9) sequencing primers
Primer sequences for sequencing of avian influenza A(H7N9) viruses that have recently emerged are also available from the Centre to National Influenza Centres only. If you are from a National Influenza Centre and would like these sequences for surveillance purposes, please send us an email at whoflu@influenzacentre.org with the subject line "NIC request for A(H7N9) primer sequences".

For more updates and information on detection of avian influenza A(H7N9):
Information from WHO on the avian influenza A(H7N9) virus
WHO real-time RT-PCR protocol for the detection of the avian A(H7N9) influenza virus